Romina Dastmalchi; Mir Davood Omrani; Mehrdokht Mazdeh; Shahram Arsang-Jang; Abolfazl Movafagh; Arezou Sayad; Mohammad Taheri
Volume 20, Issue 8 , 2018, Pages 1-6
Abstract
Background: Multiple sclerosis (MS) is an autoimmune and multifactorial disease, and its pathogenesis is associated with many genetic and environmental factors. Long Non-coding RNA (lncRNAs) are a group of genes that have recently been identified as pre- disposing genetic factors for the development ...
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Background: Multiple sclerosis (MS) is an autoimmune and multifactorial disease, and its pathogenesis is associated with many genetic and environmental factors. Long Non-coding RNA (lncRNAs) are a group of genes that have recently been identified as pre- disposing genetic factors for the development of many cancers. Objectives: This is a case–control study to evaluate the expression of two lncRNAs including Urothelial Carcinoma Associated 1 (UCA1) and Cancer-Associated Transcript 2 (CCAT2) in Relapsing-Remitting Multiple Sclerosis (RRMS) patients compared to healthy control group.Methods: In this case-control study, the expression of UCA1 and CCAT2 was evaluated in 50 RRMS patients (37 females, 13 males with a mean age of 36.2 ± 2.9 years) compared to 50 healthy controls (38 females, 12 males with a mean age of 35.3 ± 2.1), using the TaqMan real-time PCR technique. This study was conducted during 2017 and 2018 at Shahid Beheshti University of Medical Sciences, Tehran, Iran. Results: There was no significant difference between the overall expression of UCA1 (P = 0.282) and CCAT2 (P = 0.983) among the case and control groups. However, there was a significant difference in the expression of UCA1 in female patients older than 40 years incomparison with healthy age-matched females (P = 0.013). In addition, there was a significant correlation between the expression of UCA1 and CCAT2 (P < 0.0001). Conclusions: These results suggest the synergistic effects of UCA1 and CCAT2 on pathogenic aspects of MS, by affecting cellular signaling pathways such as WNT and NF-kB.
Milad Gholami; Hossein Darvish; Habib Ahmadi; Simin Rahimi-Aliabadi; Babak Emamalizadeh; Mohammad Reza Eslami Amirabadi; Javad Jamshidi; Abolfazl Movafagh
Volume 19, Issue 1 , January 2017, , Pages 1-6
Abstract
Background: Multiple sclerosis (MS) is an autoimmune disease that affects the central nervous system (CNS). MS is one of the most common cause of neurological impairment at a young age with a complex etiology. The forkhead/winged helix (FOXP3) gene encodes a transcription factor that plays an important ...
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Background: Multiple sclerosis (MS) is an autoimmune disease that affects the central nervous system (CNS). MS is one of the most common cause of neurological impairment at a young age with a complex etiology. The forkhead/winged helix (FOXP3) gene encodes a transcription factor that plays an important role in the working and progress of regulatory T cells. Loss of the FOXP3 function impairs the suppressor activity of regulatory T (T-reg) cells, which have been reported in MS patients.Objectives: To determine whether rs2232365 and rs3761548 polymorphisms of FOXP3 are associated with the risk of MS in an Iranian population.Patients and Methods: In this case-control study, a total of 384 samples consisting of 190 MS patients and 194 unrelated healthy subjects from the Iranian population were recruited between December 2014 and September 2015. The patients were diagnosed by a neurologist based on McDonald’s criteria. The control group had no history or presence of autoimmune diseases. The polymorphisms were genotyped using tetra-ARMS PCR and PCR-restriction fragment length polymorphism (RFLP) techniques.Results: The Rs2232365 G allele was significantly associated with an increased risk of MS (P = 0.0068). In contrast, the allele and genotype frequencies of rs3761548 was not significantly different between the case and control groups (P > 0.05).Conclusions: The functional variant of the FOXP3, rs2232365 A/G, may be considered a substantial risk factor for MS.
